0.4 µm Transwell Insert,PET(For 6-Well Plates)，AGCC-20406

Product Description

The 0.4 µm cell culture insert (also known as Transwell insert) is an experimental tool commonly used for cell co-culture, cell migration, and invasion assays. It is made of polyethylene terephthalate (PET) membrane with 0.4 µm pores, which allow the passage of small molecules and cell secretions but prevent cell migration (unless the cells are invasive). These inserts are typically compatible with 6-well plates and are suitable for a variety of cell biology experiments.

Product Features

1. 0.4 µm pore size: Ideal for studying cell migration, invasion, co-culture, and substance transport.

2. PET membrane: Transparent, non-toxic, and biocompatible, suitable for microscopic observation.

3. Compatible with 6-well plates: Designed to fit standard 6-well plates for convenient experimental operations.

4. Sterile packaging: Usually for single-use, ensuring a sterile environment.

Applications

1. Cell migration and invasion assays: Study the migratory and invasive abilities of tumor cells or other cell types.

2. Co-culture experiments: Investigate interactions between two different types of cells.

3. Substance transport studies: Examine the transport of small molecules, proteins, or drugs through cell monolayers.

4. Barrier function studies: Establish models such as the blood-brain barrier or intestinal barrier.

Usage Instructions (For Reference Only)

1. Prepare cells: Add cell suspension to the upper chamber of the insert and culture medium to the lower chamber.

2. Culture cells: Incubate at 37°C with 5% CO₂ to allow cells to form a monolayer on the membrane.

3. Perform experiments: Conduct migration, invasion, or transport assays based on experimental objectives.

4. Detection: Analyze results using microscopy, fluorescent staining, or biochemical methods.

Notes

1. Aseptic technique: Avoid contamination.

2. Cell density: Adjust cell seeding density according to experimental requirements.

3. Membrane treatment: Some experiments may require pre-coating the membrane with extracellular matrix (e.g., Matrigel).